Journal: Animal Models and Experimental Medicine

Article Title: Overexpression of mitofusin 2 ameliorates inflammation and oxidative stress in lipopolysaccharide‐induced mastitis model by regulating phosphofurin acidic cluster sorting protein 2

doi: 10.1002/ame2.70110

Figure Lengend Snippet: LPS (lipopolysaccharide) modulates mitochondrial‐ER (endoplasmic reticulum) membrane protein expression and disrupts MAM (mitochondria‐associated endoplasmic reticulum membrane) integrity. (A and B) Protein expression and quantification of MFN2 (mitofusin 2) in mouse mammary epithelial cells (mMEC) detected using Western blot. (C–F) Protein expression and quantification of FIS‐1 (fission protein 1), DRP‐1 (dynamin‐related protein 1), and OPA‐1 (optic atrophy 1) in mMECs detected using Western blot. (G–I) Protein expression and quantification of eIF‐2α (eukaryotic translation initiation factor 2 alpha), p‐eIF‐2α, and PACS2 (phosphofurin acidic cluster sorting protein 2) in mMECs. (J) Protein expression of MFN2 and PACS2 in mMECs after LPS stimulation detected using immunofluorescence. (K) Mitochondrial and ER marker protein PDI (protein disulfide isomerase) expression detected using laser confocal microscopy. Data are presented as mean ± SD (standard deviation).

Article Snippet: Mitochondrial fission protein 1 , 10956‐1‐AP , 1:4000 , Proteintech.

Techniques: Membrane, Expressing, Western Blot, Immunofluorescence, Marker, Confocal Microscopy, Standard Deviation

Journal: Animal Models and Experimental Medicine

Article Title: Overexpression of mitofusin 2 ameliorates inflammation and oxidative stress in lipopolysaccharide‐induced mastitis model by regulating phosphofurin acidic cluster sorting protein 2

doi: 10.1002/ame2.70110

Figure Lengend Snippet: MFN2‐PACS2 (mitofusin 2–phosphofurin acidic cluster sorting protein 2) interaction regulates MAM (mitochondria‐associated endoplasmic reticulum membrane) dynamics and mitigates LPS (lipopolysaccharide)–induced mitochondrial dysfunction. (A) CO‐IP (co‐immunoprecipitation) of MFN2 in mouse mammary epithelial cell (mMEC) lysates. (B) Immunoprecipitation of MFN2 in mMEC lysates. OE‐MFN2 denotes MFN2 overexpression. (C) Immunoprecipitation of MFN2 in mMEC lysates. (D) CO‐IP of PACS2 in mMEC lysates. (E) mRNA (messenger RNA) expression of MFN2 in mammary tissues detected using qRT‐PCR (quantitative reverse transcription polymerase chain reaction). (F) Immunoprecipitation of MFN2 in mMEC lysates. (G) Protein expression and quantification of DRP‐1 (dynamin‐related protein 1), OPA‐1 (optic atrophy 1), FIS‐1 (fission protein 1), and MFN1 in mMECs detected using Western blot. (H and I) Mitochondrial and ER marker protein PDI (protein disulfide isomerase) expression detected using laser confocal microscopy.

Article Snippet: Mitochondrial fission protein 1 , 10956‐1‐AP , 1:4000 , Proteintech.

Techniques: Membrane, Co-Immunoprecipitation Assay, Immunoprecipitation, Over Expression, RNA Expression, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Expressing, Western Blot, Marker, Confocal Microscopy